anticholinesterase potential of monoterpenoids on the whitefly bemisia tabaci and their kinetic studies

نویسندگان

k. zarred

a. laarif

ur13agr09, regional center for research in horticulture and biological agriculture (crrhab), university of sousse, tunisia. a. ben hamouda

ur13agr09, regional center for research in horticulture and biological agriculture (crrhab), university of sousse, tunisia. i. chaieb

laboratory of plant protection, national institute of agronomic research of tunisia (inrat), university of carthage, tunisia. j. mediouni-ben jemaa

چکیده

b-biotype bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. control of this insect is obstructed by resistance to many acetylcholinesterase (ache)-inhibiting insecticides, such as organophosphates and carbamates. in the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. inhibition of ache of b. tabaci was measured by colorimetric method. the results showed that all of the monoterpenoids produced ache inhibitory activity, with ic50 values ranging from 0.96 to 26.85 mm. alpha-pinene showed the most potent inhibitory activity (ic50= 0.96 mm). kinetic analysis showed reversible non-competitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. results demonstrate the ache inhibitory activity as mode of action of these monoterpenoids at relatively high concentrations. thus, this could be useful for investigation of new ecofriendly natural insecticidal compounds.

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عنوان ژورنال:
journal of agricultural science and technology

جلد ۱۹، شماره ۳، صفحات ۶۴۳-۶۵۲

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